MATLAB Source Code for Calculating Spot Centroid Position in Gray-scale Images

MATLAB source code for calculating the centroid position of a spot in grayscale images. This program implements the Otsu's method algorithm, which automatically calculates an optimal threshold to segment the spot from the background. The code performs several key operations: it loads the grayscale image, applies Otsu's thresholding to binarize the image, calculates the weighted centroid using pixel intensity values as weights, and outputs the precise (x, y) coordinates of the spot centroid. The implementation includes functions for image preprocessing, threshold calculation using histogram analysis, and centroid computation through moment calculations. This source code enables users to quickly locate spots in image processing applications and facilitates subsequent analysis and processing. By utilizing this program, users can efficiently obtain spot position information, providing strong support for further research and experiments. Additionally, the program features good extensibility - users can modify and optimize the code according to their specific requirements to adapt to different application scenarios. The modular structure allows easy integration of additional preprocessing steps or alternative thresholding methods. In summary, this MATLAB source code serves as a powerful and practical tool that helps users achieve better results and outcomes in the field of image processing.